The important things you need to know about chromatography are:
- Definition
- Working principles
- Differences and comparisons
- Calculation
- Theory
Chromatography
- separation method
- based on partitioning behaviors
- of components present of analytes
- by the moving of mobile phase
- through stationary phase
Definition (find on your own)
- chromatography
- theoretical plate
- rate theory of chromatography
Calculation (find your own. I believe you already have them)
- retention time
- capacity factor @ retention factor
- dead time
- adjusted retention time
- selectivity factor
- resolution
- equilibrium constant
- theoretical plate
- van deemter equation
- kovats index
Comparisons/ Differences
- GC vs HPLC
- SPLIT vs SPLITLESS
- PACKED COLUMN vs CAPILLARY COLUMN (including types of capillary columns)
- CARRIER GAS (including Van Deemter Plot)
Working Principles
- Flame Ionization Detector (FID)
- Electron Capture Detector (ECD)
- Thermal Conductivity Detector (TCD)
- Schematic Diagram of general GC and HPLC
Write, draw, relate and understand
Good Luck, Friends
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